Linking ecotoxicological effects on biodiversity and ecosystem functions to impairment of ecosystem services is a challenge: an illustration with the case of plant protection products.

There is growing interest in using the ecosystem services framework for environmental risk assessments of chemicals, including plant protection products (PPPs). Although this topic is increasingly discussed in the recent scientific literature, there is still a substantial gap between most ecotoxicological studies and a solid evaluation of potential ecotoxicological consequences on ecosystem services. This was recently highlighted by a collective scientific assessment (CSA) performed by 46 scientific experts who analyzed the international science on the impacts of PPPs on biodiversity, ecosystem functions, and ecosystem services. Here, we first point out the main obstacles to better linking knowledge on the ecotoxicological effects of PPPs on biodiversity and ecological processes with ecosystem functions and services. Then, we go on to propose and discuss possible pathways for related improvements. We describe the main processes governing the relationships between biodiversity, ecological processes, and ecosystem functions in response to effects of PPP, and we define categories of ecosystem functions that could be directly linked with the ecological processes used as functional endpoints in investigations on the ecotoxicology of PPPs. We then explore perceptions on the possible links between these categories of ecosystem functions and ecosystem services among a sub-panel of the scientific experts from various fields of environmental science. We find that these direct and indirect linkages still need clarification. This paper, which reflects the difficulties faced by the multidisciplinary group of researchers involved in the CSA, suggests that the current gap between most ecotoxicological studies and a solid potential evaluation of ecotoxicological consequences on ecosystem services could be partially addressed if concepts and definitions related to ecological processes, ecosystem functions, and ecosystem services were more widely accepted and shared within the ecotoxicology community. Narrowing this gap would help harmonize and extend the science that informs decision-making and policy-making, and ultimately help to better address the trade-off between social benefits and environmental losses caused by the use of PPPs.

Main conclusions and perspectives from the collective scientific assessment of the effects of plant protection products on biodiversity and ecosystem services along the land-sea continuum in France and French overseas territories.

Preservation of biodiversity and ecosystem services is critical for sustainable development and human well-being. However, an unprecedented erosion of biodiversity is observed and the use of plant protection products (PPP) has been identified as one of its main causes. In this context, at the request of the French Ministries responsible for the Environment, for Agriculture and for Research, a panel of 46 scientific experts ran a nearly 2-year-long (2020-2022) collective scientific assessment (CSA) of international scientific knowledge relating to the impacts of PPP on biodiversity and ecosystem services. The scope of this CSA covered the terrestrial, atmospheric, freshwater, and marine environments (with the exception of groundwater) in their continuity from the site of PPP application to the ocean, in France and French overseas territories, based on international knowledge produced on or transposable to this type of context (climate, PPP used, biodiversity present, etc.). Here, we provide a brief summary of the CSA's main conclusions, which were drawn from about 4500 international publications. Our analysis finds that PPP contaminate all environmental matrices, including biota, and cause direct and indirect ecotoxicological effects that unequivocally contribute to the decline of certain biological groups and alter certain ecosystem functions and services. Levers for action to limit PPP-driven pollution and effects on environmental compartments include local measures from plot to landscape scales and regulatory improvements. However, there are still significant gaps in knowledge regarding environmental contamination by PPPs and its effect on biodiversity and ecosystem functions and services. Perspectives and research needs are proposed to address these gaps.

Artificial substrata to assess ecological and ecotoxicological responses in river biofilms: Use and recommendations.

River biofilms are biological consortia of autotrophs and heterotrophs colonizing most solid surfaces in rivers. Biofilm composition and biomass differ according to the environmental conditions, having different characteristics between systems and even between river habitats. Artificial substrata (AS) are an alternative for in situ or laboratory experiments to handle the natural variability of biofilms. However, specific research goals may require decisions on colonization time or type of substrata. Substrata properties (i.e., texture, roughness, hydrophobicity) and the colonization period and site are selective factors of biofilm characteristics. Here we describe the uses of artificial substrata in the assessment of ecological and ecotoxicological responses and propose a decision tree for the best use of artificial substrata in river biofilm studies. We propose departing from the purpose of the study to define the necessity of obtaining a realistic biofilm community, from which it may be defined the colonization time, the colonization site, and the type of artificial substratum. Having a simple or mature biofilm community should guide our decisions on the colonization time and type of substrata to be selected for the best use of AS in biofilm studies. Tests involving contaminants should avoid adsorbing materials while those ecologically oriented may use any AS mimicking those substrata occurring in the streambed.•We review the utilization of different artificial substrata to colonize biofilm in river ecology and ecotoxicology.•We propose a decision tree to guide on selecting the appropriate artificial substrata and colonization site and duration.•Type of artificial substrata (material, size, shape...) and colonization duration are to be decided according to the specific purpose of the study.

Resistance but not recovery is related to the role of specialist taxa in river communities submitted to hydric stress.

One of the main effects of global change is the human interference in the global water cycle, which alters river hydrological dynamics and submits their biological communities to hydric stress. Hydric stress is a pulse disturbance with potential multiple effects on biodiversity and functions in river ecosystems. The presence of habitat specialists may support the response of biological communities to pulse disturbances, maintaining ecological functions more consistently than other communities only having generalists. We tested this general hypothesis in stream communities submitted to increasing hydric stress (normal conditions vs humidity vs desiccation). We used communities with variable proportion of specialist algal and cyanobacterial taxa and tested their resistance to hydric stress by analyzing potential changes on their number of species, biovolume, proportion of intact cells, and photosynthetic variables (basal fluorescence, photosynthetic yield). We also evaluated the recovery of ecological functions (net community primary production, community respiration, phosphorus uptake) once hydric stress conditions ended. Hydric stress caused a slight decrease in the number of species and biovolume of assemblages, but the proportion of intact cells did not significantly change because of the disturbance. Basal fluorescence and photosynthetic yield under hydric stress decreased more markedly in communities without specialist taxa, while communities with habitat specialists resisted better. Metabolism did not remarkably decrease under moderate hydric stress, but dropped by half under desiccation in all communities, having or not specialist taxa. Overall, specialist taxa did provide higher resistance to stress but did not support a distinct recovery of ecological functions. We suggest that this characteristic response is related to the high plasticity of biofilm structures.

Extreme weather events threaten biodiversity and functions of river ecosystems: evidence from a meta-analysis.

Both gradual and extreme weather changes trigger complex ecological responses in river ecosystems. It is still unclear to what extent trend or event effects alter biodiversity and functioning in river ecosystems, adding considerable uncertainty to predictions of their future dynamics. Using a comprehensive database of 71 published studies, we show that event - but not trend - effects associated with extreme changes in water flow and temperature substantially reduce species richness. Furthermore, event effects - particularly those affecting hydrological dynamics - on biodiversity and primary productivity were twice as high as impacts due to gradual changes. The synthesis of the available evidence reveals that event effects induce regime shifts in river ecosystems, particularly affecting organisms such as invertebrates. Among extreme weather events, dryness associated with flow interruption caused the largest effects on biota and ecosystem functions in rivers. Effects on ecosystem functions (primary production, organic matter decomposition and respiration) were asymmetric, with only primary production exhibiting a negative response to extreme weather events. Our meta-analysis highlights the disproportionate impact of event effects on river biodiversity and ecosystem functions, with implications for the long-term conservation and management of river ecosystems. However, few studies were available from tropical areas, and our conclusions therefore remain largely limited to temperate river systems. Further efforts need to be directed to assemble evidence of extreme events on river biodiversity and functioning.

Energy limitation or sensitive predators? Trophic and non-trophic impacts of wastewater pollution on stream food webs.

Impacts of environmental stressors on food webs are often difficult to predict because trophic levels can respond in divergent ways, and biotic interactions may dampen or amplify responses. Here we studied food-web-level impacts of urban wastewater pollution, a widespread source of degradation that can alter stream food webs via top-down and bottom-up processes. Wastewater may (1) subsidize primary producers by decreasing nutrient limitation, inducing a wide-bottomed trophic pyramid. However, (2) wastewater may also reduce the quality and diversity of resources, which could decrease energy transfer efficiency by reducing consumer fitness, leading to predator starvation. Additionally, (3) if higher trophic levels are particularly sensitive to pollution, primary consumers could be released from predation pressure. We tested these hypotheses in 10 pairs of stream sites located upstream and downstream of urban wastewater effluents with different pollutant levels. We found that wastewater pollution reduced predator richness by ∼34%. Community size spectra (CSS) slopes were steeper downstream than upstream of wastewater effluents in all except one impact site where predators became locally extinct. Further, variation in downstream CSS slopes were correlated with pollution loads: the more polluted the stream, the steeper the CSS. We estimate that wastewater pollution decreased energy transfer efficiencies to primary consumers by ∼70%, limiting energy supply to predators. Additionally, traits increasing vulnerability to chemical pollution were overrepresented among predators, which presented compressed trophic niches (δ15 N-δ13 C) downstream of effluents. Our results show that wastewater pollution can impact stream food webs via a combination of energy limitation to consumers and extirpation of pollution-sensitive top predators. Understanding the indirect (biotically mediated) vs. direct (abiotic) mechanisms controlling responses to stress may help anticipating impacts of altered water quantity and quality, key signatures of global change.

Green and brown stream trophic food chains show specific responses to constant or hump-shaped inputs of copper.

The brown food chain (based on decomposers) co-exists in streams with the green food chain (based on primary producers). The two trophic chains perform specific ecosystem functions which may be altered by the effect of contaminants. Copper is a common contaminant with recognized effects on several compartments of the two trophic chains. We applied it in two separate mesocosm experiments, in which we tested the effects of copper after contrasting patterns of contaminant exposure (constant vs hump-shaped). The constant input simulated a chronic contamination (average of 20 µg/L Cu), while the hump-shaped simulated the steady arrival of copper, the occurrence of a peak (reaching ca. 60 µg/L Cu), and its progressive decrease (down to 10-15 µg/L Cu). In the green trophic food chain, copper exposure decreased the total chlorophyll-a as well as the basal fluorescence and the photosynthetic yield. The treatment receiving hump-shaped inputs caused the highest mortality of the green food chain consumer, the snail Radix balthica. In the chronic copper exposure, mortality achieved a maximum of 80% by the end of the experiment but occurred later than that in the hump-shaped treatment. Effects on the brown food chain were not so pronounced; the microbial decomposition rate of leaflitter decreased nearly ca. 50% after 14 days of copper exposure. Effects on decomposition translated into the ingestion performance of detritivores, which decreased in the two copper treatments. Our results provide evidence that copper affected the two trophic food chains. The hump-shaped arrival included a peak of high concentration, which caused lethal effects on the consumers, but also a decreasing limb, which allowed a partial recovery of the algal photosynthetic variables. Our results suggest the need to consider the different compartments and functions performed within the stream trophic web when evaluating the effects of a contaminant in a river ecosystem.

The future for Mediterranean wetlands: 50 key issues and 50 important conservation research questions.

Wetlands are critically important for biodiversity and human wellbeing, but face a range of challenges. This is especially true in the Mediterranean region, where wetlands support endemic and threatened species and remain integral to human societies, but have been severely degraded in recent decades. Here, in order to raise awareness of future challenges and opportunities for Mediterranean wetlands, and to inform proactive research and management, we identified (a) 50 key issues that might affect Mediterranean wetlands between 2020 and 2050, and (b) 50 important research questions that, if answered, would have the greatest impact on the conservation of Mediterranean wetlands between 2020 and 2050. We gathered ideas through an online survey and review of recent literature. A diverse assessment panel prioritised ideas through an iterative, anonymised, Delphi-like process of scoring, voting and discussion. The prioritised issues included some that are already well known but likely to have a large impact on Mediterranean wetlands in the next 30 years (e.g. the accumulation of dams and reservoirs, plastic pollution and weak governance), and some that are currently overlooked in the context of Mediterranean wetlands (e.g. increasing desalination capacity and development of antimicrobial resistance). Questions largely focused on how best to carry out conservation interventions, or understanding the impacts of threats to inform conservation decision-making. This analysis will support research, policy and practice related to environmental conservation and sustainable development in the Mediterranean, and provides a model for similar analyses elsewhere in the world. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10113-020-01743-1.

Duration of water flow interruption drives the structure and functional diversity of stream benthic diatoms.

Flow cessation affects river ecosystems submitted to low precipitation and increased water demand, and creates unfavourable conditions to aquatic biological communities. Diatoms are amongst the most sensitive biological groups to hydric stress, making them good indicators of preceding hydrological conditions. We here analyse the response of diatom assemblages to the duration and frequency of non-flow periods in 23 Mediterranean temporary streams. All of them experienced a strong decrease in water flow during summer, leading to a period of flow cessation. In addition, other ten permanent streams (zero dry days during the study period) were included in the study for comparative purposes. Temporary and permanent streams showed similar diatom species richness, evenness, and alpha diversity. However, beta diversity was higher in temporary than permanent streams, regardless rare taxa (<1% occurrence) had similar numbers in the two types of streams. Alpha diversity, richness, and evenness of diatom assemblages in the temporary streams changed with the duration of the non-flow period. Durations of 50-100 days were associated to higher alpha diversity, richness, and evenness, but longer non-flow periods caused their decrease. Diatoms thriving under the most extreme conditions were mostly aerophilic, pioneer, and motile taxa. The proportion of aerophilic diatom taxa increased beyond 100 dry days, particularly in those sites receiving more intense solar radiation. Overall, the taxonomical and functional composition of diatom assemblages mostly responded to the duration of the non-flow period, irrespectively of these being consecutive or not. This study shows that diatom assemblages from temporary streams contain taxa adapted to non-flow conditions, but remain vulnerable to further reductions of water flow associated to climate or global change. 'Unimpacted' watercourses in the Mediterranean region, independently of their flow regime, should be urgently protected and used as taxonomical and functional early indicators of climate change.

Framing biophysical and societal implications of multiple stressor effects on river networks.

Urbanization, agriculture, and the manipulation of the hydrological cycle are the main drivers of multiple stressors affecting river ecosystems across the world. Physical, chemical, and biological stressors follow characteristic patterns of occurrence, intensity, and frequency, linked to human pressure and socio-economic settings. The societal perception of stressor effects changes when moving from broad geographic regions to narrower basin or waterbody scales, as political and ecologically based perspectives change across scales. Current approaches relating the stressor effects on river networks and human societies fail to incorporate complexities associated to their co-occurrence, such as: i) the evidence that drivers can be associated to different stressors; ii) their intensity and frequency may differ across spatial and temporal scales; iii) their differential effects on biophysical receptors may be related to their order of occurrence; iv) current and legacy stressors may produce unexpected outcomes; v) the potentially different response of different biological variables to stressor combinations; vi) the conflicting effects of multiple stressors on ecosystem services; and, vii) management of stressor effects should consider multiple occurrence scales. We discuss how to incorporate these aspects to present frameworks considering biophysical and societal consequences of multiple stressors, to better understand and manage the effects being caused on river networks.

Combined effects of urban pollution and hydrological stress on ecosystem functions of Mediterranean streams.

Urban pollution and hydrological stress are common stressors of stream ecosystems, but their combined effects on ecosystem functioning are still unclear. We measured a set of functional processes and accompanying environmental variables in locations upstream and downstream of urban sewage inputs in 13 streams covering a wide range of water pollution levels and hydrological variability. Sewage inputs seriously impaired stream chemical characteristics and led to complex effects on ecosystem functioning. Biofilm biomass accrual, whole-reach nutrient uptake and metabolism (ecosystem respiration) were generally subsidized, whereas organic matter decomposition and biofilm phosphorus uptake capacity decreased with increasing pollutant concentrations. Hydrological stress affected stream ecosystem functioning but its effect was minor compared to the effects of urban pollution, due to the large inter-site variability of the streams. Changes appeared mainly linked to the concentration of pharmaceutically active compounds, followed by other chemical characteristics and by hydrology. The results point to the need to further improve sewage treatment, especially as climate change will stress riverine organisms and reduce the dilution capacity of the receiving streams.

Occurrence of regulated pollutants in populated Mediterranean basins: Ecotoxicological risk and effects on biological quality.

Chemical stressors co-occur in mixtures into watercourses and this complicates predicting their effects on their ecological status. Our knowledge of river basin specific pollutants (RBSPs) is still limited, but it remains necessary to ensure the good chemical and ecological status. We performed an exercise on Mediterranean river sites exposed to urban and industrial pressures in order to, i) prioritize the occurring chemicals, ii) assessing the site's specific chemical risk (RQsite), and iii) relating the chemical risk to the biological quality, using as evidences invertebrates and diatom indices. Mediterranean rivers suffer from strong pressures which lead to a poor dilution ability, which makes the inhabiting biota highly vulnerable. The most frequent pollutants in the 89 sites surveyed included pharmaceutical products such as the antibiotics azithromycin, clarithromycin, and erythromycin, and the anti-inflammatory diclofenac, and products of industrial origin such as perfluorinated PFOS, nickel, and nonylphenol. Both the diatom index IPS and the macroinvertebrate index IBMWP hold strong negative correlations to RQsite, indicating a significant contribution of chemicals to biological impairment. Chemical contaminants (but not nutrients or dissolved organic carbon) were associated with significant changes to the taxonomic composition of invertebrate communities, but not to that of diatom communities. Our analyses indeed reveal that the impact of co-occurring chemicals translates onto negative effects in the biological quality. Our approach may be of use to evidence impacts on water resources and water quality in rivers under strong human pressure.

Lifestyle preferences drive the structure and diversity of bacterial and archaeal communities in a small riverine reservoir.

Spatial heterogeneity along river networks is interrupted by dams, affecting the transport, processing, and storage of organic matter, as well as the distribution of biota. We here investigated the structure of planktonic (free-living, FL), particle-attached (PA) and sediment-associated (SD) bacterial and archaeal communities within a small reservoir. We combined targeted-amplicon sequencing of bacterial and archaeal 16S rRNA genes in the DNA and RNA community fractions from FL, PA and SD, followed by imputed functional metagenomics, in order to unveil differences in their potential metabolic capabilities within the reservoir (tail, mid, and dam sections) and lifestyles (FL, PA, SD). Both bacterial and archaeal communities were structured according to their life-style preferences rather than to their location in the reservoir. Bacterial communities were richer and more diverse when attached to particles or inhabiting the sediment, while Archaea showed an opposing trend. Differences between PA and FL bacterial communities were consistent at functional level, the PA community showing higher potential capacity to degrade complex carbohydrates, aromatic compounds, and proteinaceous materials. Our results stressed that particle-attached prokaryotes were phylogenetically and metabolically distinct from their free-living counterparts, and that performed as hotspots for organic matter processing within the small reservoir.

Unravelling the effects of multiple stressors on diatom and macroinvertebrate communities in European river basins using structural and functional approaches.

Rivers suffer from more severe decreases in species diversity compared to other aquatic and terrestrial ecosystems due to a variety of pressures related to human activities. Species provide different roles in the functioning of the ecosystem, and their loss may reduce the capacity of the ecosystems to respond to multiple stressors. The effects on diversity will differ based on the type, combination and severity of stressors, as well as on the characteristics of the community composition and tolerance. Multiple trait-based approaches (MTBAs) can help to unravel the effects of multiple stressors on communities, providing a mechanistic interpretation, and, thus, complementing traditional biodiversity assessments using community structure. We studied the relationships between diversity indexes and trait composition of macroinvertebrate and diatom communities, as well as environmental variables that described the hydrological and geomorphological alterations and toxic pollution (pesticides and pharmaceuticals) of three different European river basins: the Adige, the Sava, and the Evrotas. These river basins can be considered representative cases of different situations in European freshwater systems. Hydrological variables were the main drivers determining the community structure and function in the rivers, for both diatoms and macroinvertebrates. For diatom communities, pharmaceutical active compound (PhAC) toxic units were also identified as a very important driver of diversity changes, explaining up to 57% of the variance in taxonomic richness. For macroinvertebrates, river geomorphology was an important driver of structural changes, particularly affecting Plecoptera richness. In addition, PhAC and pesticide toxic units were also identified as stressors for macroinvertebrate communities. MTBA provided a detailed picture of the effects of the stressors on the communities and confirmed the importance of hydrological variables in shaping the functional attributes of the communities.

Ecoregional Characteristics Drive the Distribution Patterns of Neotropical Stream Diatoms.

We assessed the relative influence of ecoregional features in explaining diatom distribution in the Orinoco river basin. Ecoregions in the Colombian Orinoco can be seen as imprints of the evolutionary history of the basin, for their current biodiversity and physiographic features are the result of the geological and climatic shifts that have occurred since the Tertiary. Thus, they represent an ideal testing ground for studying the interplay between ecological and evolutionary processes shaping diversity patterns of microorganisms, such as diatoms, in the present day. To study this interplay, we compared diatom community composition variance within and among seven ecoregions and assessed the explanatory power of environmental, spatial and historical drivers. This was done by a combination of correlation analyses, multivariate methods and constrained ordinations. We also deconstructed the whole community data set into ecological guilds (low- and high-profile, and motile) to explore their individual response to the contemporary and historical drivers. Taken together, these analyses indicated that contemporary constraints to species occurrence and dispersal, as well as the legacies of historical events, can provide an explanation for the contemporary distribution of diatoms in the Colombian Orinoco. Specifically, we provided evidence showing that both historical legacies and contemporary environmental conditions (temperature, pH, and phosphorus concentration) are interacting to determine diatoms' distribution. Our results suggest the need to consider ecoregional gradients for unraveling the mechanisms shaping tropical diversity as well as for designing conservation plans.

Multiple Stressors Determine Community Structure and Estimated Function of River Biofilm Bacteria.

Freshwater ecosystems are exposed to multiple stressors, but their individual and combined effects remain largely unexplored. Here, we investigated the response of stream biofilm bacterial communities to warming, hydrological stress, and pesticide exposure. We used 24 artificial streams on which epilithic (growing on coarse sediments) and epipsammic (growing on fine sediments) stream biofilms were maintained. Bacterial community composition and estimated function of biofilms exposed during 30 days to individual and combined stressors were assessed using 16S rRNA gene metabarcoding. Among the individual effects by stressors, hydrological stress (i.e., a simulated low-flow situation) was the most relevant, since it significantly altered 57% of the most abundant bacterial taxa (n = 28), followed by warming (21%) and pesticide exposure (11%). Regarding the combined effects, 16% of all stressor combinations resulted in significant interactions on bacterial community composition and estimated function. Antagonistic responses prevailed (57 to 89% of all significant interactions), followed by synergisms (11 to 43%), on specific bacterial taxa, indicating that multiple-stressor scenarios could lead to unexpected shifts in the community composition and associated functions of riverine bacterial communities.IMPORTANCE Freshwater ecosystems such as rivers are of crucial importance for human well-being. However, human activities result in many stressors (e.g., toxic chemicals, increased water temperatures, and hydrological alterations) cooccurring in rivers and streams worldwide. Among the many organisms inhabiting rivers and streams, bacteria are ecologically crucial; they are placed at the base of virtually all food webs and they recycle the organic matter needed for bigger organisms. Most of these bacteria are in close contact with river substratum, where they form the biofilms. There is an urgent need to evaluate the effects of these stressors on river biofilms, so we can anticipate future environmental problems. In this study, we experimentally exposed river biofilms to a pesticide mixture, an increase in water temperature and a simulated low-flow condition, in order to evaluate the individual and joint effects of these stressors on the bacterial community composition and estimated function.

Bioconcentration and bioaccumulation of C60 fullerene and C60 epoxide in biofilms and freshwater snails (Radix sp.).

Fullerenes are carbon nanomaterials that have awaken a strong interest due to their adsorption properties and potential applications in many fields. However, there are some gaps of information about their effects and bioconcentration potential in the aquatic biota. In the present work, freshwater biofilms and snails (Radix sp.) were exposed to fullerene C60 aggregates, at concentrations in the low µg/L order, in mesocosms specifically designed to mimic the conditions of a natural stream. The bioconcentration factors of C60 fullerene and its main transformation product, [6,6]C60O epoxide, were studied to the mentioned organisms employing analyses by liquid chromatography coupled to high-resolution mass spectrometry. Our results show that C60 fullerene and its [6,6]C60O present a low bioconcentration factor (BCF) to biofilms: BCFC60 = 1.34 ±â€¯0.95 L/kgdw and BCFC60O = 1.43 ±â€¯0.72 L/kgdw. This suggests that the sorption of these aggregates to biota may be less favoured than it would be suggested by its hydrophobic character. According to our model, the surface of fullerene aggregates is saturated with [6,6]C60O molecules, which exposes the polar epoxide moieties in the surface of the aggregates and decreases their affinity to biofilms. In contrast, freshwater snails showed a moderate capacity to actively retain C60 fullerenes in their organism (BAFC60 = 2670 ±â€¯3070 L/kgdw; BAFC60O = 1330 ±â€¯1680 L/kgdw), probably through ingestion. Our results indicate that the bioaccumulation of these carbon nanomaterials can be hardly estimated using their respective octanol-water partition coefficients, and that their colloidal properties, as well as the feeding strategies of the tested organism, play fundamental roles.

Aquatic macroinvertebrates under stress: Bioaccumulation of emerging contaminants and metabolomics implications.

The current knowledge on bioaccumulation of emerging contaminants (ECs) in aquatic invertebrates exposed to the realistic environmental concentrations is limited. Even less is known about the effects of chemical pollution exposure on the metabolome of aquatic invertebrates. We conducted an in situ translocation experiment with passive filter-feeding caddisfly larvae (Hydropsyche sp.) in an effluent-influenced river in order to i) unravel the bioaccumulation (and recovery) dynamics of ECs in aquatic invertebrates, and ii) test whether exposure to environmentally realistic concentrations of ECs will translate into metabolic profile changes in the insects. The experiment was carried out at two sites, upstream and downstream of the discharge of an urban wastewater treatment plant effluent. The translocated animals were collected at 2-week intervals for 46 days. Both pharmaceuticals and endocrine disrupting compounds (EDCs) were detected in water (62 and 7 compounds, respectively), whereas in Hydropsyche tissues 5 EDCs accumulated. Overall, specimens from the upstream site translocated to the impacted site reached higher ECs concentrations in their tissues, as a reflection of the contaminants' water concentrations. However, bioaccumulation was a temporary process susceptible to change under lower contaminant concentrations. Non-targeted metabolite profiling detected fine metabolic changes in translocated Hydropsyche larvae. Both translocations equally induced stress, but it was higher in animals translocated to the impacted site.

Effects of multiple stressors on river biofilms depend on the time scale.

Global change exposes ecosystems to a myriad of stressors differing in their spatial (i.e. surface of stressed area) and temporal (i.e. exposure time) components. Among freshwater ecosystems, rivers and streams are subject to physical, chemical and biological stressors, which interact with each other and might produce diverging effects depending on exposure time. We conducted a manipulative experiment using 24 artificial streams to examine the individual and combined effects of warming (1.6 °C increase in water temperature), hydrological stress (simulated low-flow situation) and chemical stress caused by pesticide exposure (15.1-156.7 ng L-1) on river biofilms. We examined whether co-occurring stressors could lead to non-additive effects, and if these differed at two different exposure times. Specifically, structural and functional biofilm responses were assessed after 48 hours (short-term effects) and after 30 days (long-term effects) of exposure. Hydrological stress caused strong negative impacts on river biofilms, whereas effects of warming and pesticide exposure were less intense, although increasing on the long term. Most stressor combinations (71%) resulted in non-significant interactions, suggesting overall additive effects, but some non-additive interactions also occurred. Among non-additive interactions, 59% were classified as antagonisms after short-term exposure to the different stressor combinations, rising to 86% at long term. Our results indicate that a 30-day exposure period to multiple stressors increases the frequency of antagonistic interactions compared to a 48-hour exposure to the same conditions. Overall, the impacts of multiple-stressor occurrences appear to be hardly predictable from individual effects, highlighting the need to consider temporal components such as duration when predicting the effects of multiple stressors.

Effects of olive mill wastewater discharge on benthic biota in Mediterranean streams.

Olive mill wastewaters (OMW) discharging in river ecosystems cause significant adverse effects on their water chemistry and biological communities. We here examined the effects of OMW loads in four streams of a Mediterranean basin characterized by changing flow. The diatom and macroinvertebrate community structures were compared between upstream (control) and downstream (impacted) sites receiving OMW discharge. We also tested if effects occurred at the organism level, i.e. the occurrence of deformities in diatom valves, and the sediment toxicity on the midge Chironomus riparius. We evaluated these effects through a two-year analysis, at various levels of chemical pollution and dilution capacity. The impacted sites had high phenol concentrations and organic carbon loads during and after olive mill (OM) operation, and were characterized by higher abundances of pollution-tolerant diatom and macroinvertebrate taxa. Diatom valve deformities occurred more frequently at the impacted sites. The development of C. riparius was affected by phenolic compounds and organic carbon concentrations in the sediments. The similarity in the diatom and macroinvertebrate assemblages between control and impacted sites decreased at lower flows. Diatoms were more sensitive in detecting deterioration in the biological status of OMW receiving waterways than macroinvertebrates. Our results indicate that the negative effects of OMW extended to the whole benthic community, at both assemblage and organism level.